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Understanding the mechanism of red light-induced melatonin biosynthesis facilitates the engineering of melatonin-enriched tomatoes.

ABSTRACT: Melatonin is a functionally conserved broad-spectrum physiological regulator found in most biological organisms in nature. Enrichment of tomato fruit with melatonin not only enhances its agronomic traits but also provides extra health benefits. In this study, we elucidate the full melatonin biosynthesis pathway in tomato fruit by identifying biosynthesis-related genes that encode caffeic acid O-methyltransferase 2 (SlCOMT2) and N-acetyl-5-hydroxytryptamine-methyltransferases 5/7 (SlASMT5/7). We further reveal that red light supplementation significantly enhances the melatonin content in tomato fruit. This induction relies on the "serotonin-N-acetylserotonin-melatonin" biosynthesis route via the SlphyB2-SlPIF4-SlCOMT2 module. Based on the regulatory mechanism, we design a gene-editing strategy to target the binding motif of SlPIF4 in the promoter of SlCOMT2, which significantly enhances the production of melatonin in tomato fruit. Our study provides a good example of how the understanding of plant metabolic pathways responding to environmental factors can guide the engineering of health-promoting foods.

SUBMITTER: Zhang Z

PROVIDER: S-EPMC10491657 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature

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Understanding the mechanism of red light-induced melatonin biosynthesis facilitates the engineering of melatonin-enriched tomatoes.

Zhang Zixin Z Zhang Xin X Chen Yuting Y Jiang Wenqian W Zhang Jing J Wang Jiayu J Wu Yanjun Y Wang Shouchuang S Yang Xiao X Liu Mingchun M Zhang Yang Y

Nature communications 20230908 1

Melatonin is a functionally conserved broad-spectrum physiological regulator found in most biological organisms in nature. Enrichment of tomato fruit with melatonin not only enhances its agronomic traits but also provides extra health benefits. In this study, we elucidate the full melatonin biosynthesis pathway in tomato fruit by identifying biosynthesis-related genes that encode caffeic acid O-methyltransferase 2 (SlCOMT2) and N-acetyl-5-hydroxytryptamine-methyltransferases 5/7 (SlASMT5/7). We ...[more]

PMID: 37684283

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Project description:Greenhouse crop production in northern countries often relies heavily on supplemental lighting for year-round yield and product quality. Among the different spectra used in supplemental lighting, red is often considered the most efficient, but plants do not develop normally when grown solely under monochromatic red light ("red light syndrome"). Addition of blue light has been shown to aid normal development, and typical lighting spectra in greenhouse production include a mixture of red and blue light. However, it is unclear whether sunlight, as part of the light available to plants in the greenhouse, may be sufficient as a source of blue light. In a greenhouse high-wire tomato (Solanum lycopersicum), we varied the percentage of blue supplemental light (in a red background) as 0, 6, 12, and 24%, while keeping total photosynthetically active radiation constant. Light was supplied as a mixture of overhead (99 μmol m-2 s-1) and intracanopy (48 μmol m-2 s-1) LEDs, together with sunlight. Averaged over the whole experiment (111 days), sunlight comprised 58% of total light incident onto the crop. Total biomass, yield and number of fruits increased with the addition of blue light to an optimum, suggesting that both low (0%) and high (24%) blue light intensities were suboptimal for growth. Stem and internode lengths, as well as leaf area, decreased with increases in blue light percentage. While photosynthetic capacity increased linearly with increases in blue light percentage, photosynthesis in the low blue light treatment (0%) was not low enough to suggest the occurrence of the red light syndrome. Decreased biomass at low (0%) blue light was likely caused by decreased photosynthetic light use efficiency. Conversely, decreased biomass at high (24%) blue light was likely caused by reductions in canopy light interception. We conclude that while it is not strictly necessary to add blue light to greenhouse supplemental red light to obtain a functional crop, adding some (6-12%) blue light is advantageous for growth and yield while adding 24% blue light is suboptimal for growth.

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Understanding the mechanism of red light-induced melatonin biosynthesis facilitates the engineering of melatonin-enriched tomatoes.

Publications

Understanding the mechanism of red light-induced melatonin biosynthesis facilitates the engineering of melatonin-enriched tomatoes.

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