Peroxisomal compartmentalization of amino acid biosynthesis reactions imposes an upper limit on compartment size.

ABSTRACT: Cellular metabolism relies on just a few redox cofactors. Selective compartmentalization may prevent competition between metabolic reactions requiring the same cofactor. Is such compartmentalization necessary for optimal cell function? Is there an optimal compartment size? Here we probe these fundamental questions using peroxisomal compartmentalization of the last steps of lysine and histidine biosynthesis in the fission yeast Schizosaccharomyces japonicus. We show that compartmentalization of these NAD⁺ dependent reactions together with a dedicated NADH/NAD⁺ recycling enzyme supports optimal growth when an increased demand for anabolic reactions taxes cellular redox balance. In turn, compartmentalization constrains the size of individual organelles, with larger perox

SUBMITTER: Gu Y 

PROVIDER: S-EPMC10491753 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature