Intracellular BAPTA directly inhibits PFKFB3, thereby impeding mTORC1-driven Mcl-1 translation and killing MCL-1-addicted cancer cells.

ABSTRACT: Intracellular Ca²⁺ signals control several physiological and pathophysiological processes. The main tool to chelate intracellular Ca²⁺ is intracellular BAPTA (BAPTA_i), usually introduced into cells as a membrane-permeant acetoxymethyl ester (BAPTA-AM). Previously, we demonstrated that BAPTA_i enhanced apoptosis induced by venetoclax, a BCL-2 antagonist, in diffuse large B-cell lymphoma (DLBCL). This finding implied a novel interplay between intracellular Ca²⁺ signaling and anti-apoptotic BCL-2 function. Hence, we set out to identify the underlying mechanisms by which BAPTA_i enhances cell death in B-cell cancers. In this study, we discovered that BAPTA_i alone induced apoptosis in hematological cancer cell lines that

SUBMITTER: Sneyers F 

PROVIDER: S-EPMC10491774 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature