Analysis of structural effects of sickle cell disease on brain vasculature of mice using three-dimensional quantitative phase imaging.

ABSTRACT:

Significance

Although the molecular origins of sickle cell disease (SCD) have been extensively studied, the effects of SCD on the vasculature-which can influence blood clotting mechanisms, pain crises, and strokes-are not well understood. Improving this understanding can yield insight into the mechanisms and wide-ranging effects of this devastating disease.

Aim

We aim to demonstrate the ability of a label-free 3D quantitative phase imaging technology, called quantitative oblique back-illumination microscopy (qOBM), to provide insight into the effects of SCD on brain vasculature.

Approach

Using qOBM, we quantitatively analyze the vasculature of freshly excised, but otherwise unaltered, whole mouse brains. We use Townes sickle transgenic mice, which closely recapitulate

SUBMITTER: Filan C 

PROVIDER: S-EPMC10491933 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature