Genome-wide map of R-loops reveals its interplay with transcription and genome integrity during germ cell meiosis.

ABSTRACT:

Introduction

The R-loop is a naturally formed three-strand nucleic acid structure that recently has been reported to participate in multiple biological processes and helped answer some previously unexplained scientific questions. Meiosis process involves multiple chromatin-related events such as DNA double-stranded breaks (DSB) formation, repairing and transcriptional dynamics.

Objectives

Explore the regulatory roles and physiological functions of R-loops in the mammalian meiosis process.

Methods

In our study, using genome-wide S9.6 CUT & Tag seq, we first mapped the genomic distribution and dynamic changes of R-loop during the meiotic process in mice, from spermatogonia to secondary spermatocytes. And we further explore the role of R-loop in physiological conditions

SUBMITTER: Jiang Y 

PROVIDER: S-EPMC10491972 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature