Development of RNA/DNA automated extraction and purification device for infectious disease diagnosis.

ABSTRACT: Genetic tests using RNA/DNA are the most accurate for diagnosing infectious diseases and assessing disease susceptibility, including COVID-19. However, manual specimen handling and the risk of secondary infections by medical staff highlight the need for automated equipment. Automation methods, such as bead purification, have limitations with high-viscosity specimens, while column purification requires complex equipment. This study aimed to develop an automated device using the column purification method for safe and reliable infectious disease diagnosis. We compared the yield and purification of three nucleic acid extraction methods (centrifugation, pressurization, and depressurization) and examined the adaptation of the extraction methods to automated device. Furthermore, we examined the

SUBMITTER: Mukae K 

PROVIDER: S-EPMC10492190 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature