Natural diversity of lactococci in γ-aminobutyric acid (GABA) production and genetic and phenotypic determinants.

ABSTRACT:

Background

γ-aminobutyric acid (GABA) is a bioactive compound produced by lactic acid bacteria (LAB). The diversity of GABA production in the Lactococcus genus is poorly understood. Genotypic and phenotypic approaches were therefore combined in this study to shed light on this diversity. A comparative genomic study was performed on the GAD-system genes (gadR, gadC and gadB) involved in GABA production in 36 lactococci including L. lactis and L. cremoris species. In addition, 132 Lactococcus strains were screened for GABA production in culture medium supplemented with 34 mM L-glutamic acid with or without NaCl (0.3 M).

Results

Comparative analysis of the nucleotide sequence alignments revealed the same genetic organization of the GAD system in all strains except one, which ha

SUBMITTER: Laroute V 

PROVIDER: S-EPMC10492284 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature