Fluid shear stress induces a shift from glycolytic to amino acid pathway in human trophoblasts.

ABSTRACT:

Background

The human placenta, a tissue with a lifespan limited to the period of pregnancy, is exposed to varying shear rates by maternal blood perfusion depending on the stage of development. In this study, we aimed to investigate the effects of fluidic shear stress on the human trophoblast transcriptome and metabolism.

Results

Based on a trophoblast cell line cultured in a fluidic flow system, changes caused by shear stress were analyzed and compared to static conditions. RNA sequencing and bioinformatics analysis revealed an altered transcriptome and enriched gene ontology terms associated with amino acid and mitochondrial metabolism. A decreased GLUT1 expression and reduced glucose uptake, together with downregulated expression of key glycolytic rate-limiting enzymes, he

SUBMITTER: Brugger BA 

PROVIDER: S-EPMC10492287 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature