Integrated bioinformatics analysis of dendritic cells hub genes reveal potential early tuberculosis diagnostic markers.

ABSTRACT:

Background

Dendritic cells (DCs) are most potent antigen-processing cells and play key roles in host defense against Mycobacterium tuberculosis (MTB) infection. In this study, hub genes in DCs during MTB infection were first investigated using bioinformatics approaches and further validated in Monocyte-derived DCs.

Methods

Microarray datasets were obtained from Gene Expression Omnibus (GEO) database. Principal component analysis (PCA) and immune infiltration analysis were performed to select suitable samples for further analysis. Differential analysis and functional enrichment analysis were conducted on DC samples, comparing live MTB-infected and non-infected (NI) groups. The CytoHubba plugin in Cytoscape was used to identify hub genes from the differentially expressed genes

SUBMITTER: Wu X 

PROVIDER: S-EPMC10492340 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature