Characterization of the soft-tissue wall lining residual periodontal pockets and implications in periodontal wound healing.

ABSTRACT:

Aim

To characterize the soft-tissue wall of remaining periodontal pockets for wound healing-related parameters versus healthy gingival crevices in the same individuals.

Materials and methods

Gingival tissues collected from the diseased interface of pockets (GT biopsies) and from healthy gingival crevices (G biopsies) were subjected to RT²-profiler PCR Array for wound healing-related markers and network analysis of differentially expressed genes. Lymphangiogenesis-related gene expression was determined by qRT-PCR. The migration potential of mesenchymal stem cells isolated from GT biopsies (GT-MSCs) and G biopsies (G-MSCs) was evaluated by the scratch- and the transwell migration assays. The total collagen protein content was determined in GT-MSCs and G-MSCs homogen

SUBMITTER: Gousopoulou E 

PROVIDER: S-EPMC10492763 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature