Griddient: a microfluidic array to generate reconfigurable gradients on-demand for spatial biology applications.

ABSTRACT: Biological tissues are highly organized structures where spatial-temporal gradients (e.g., nutrients, hypoxia, cytokines) modulate multiple physiological and pathological processes including inflammation, tissue regeneration, embryogenesis, and cancer progression. Current in vitro technologies struggle to capture the complexity of these transient microenvironmental gradients, do not provide dynamic control over the gradient profile, are complex and poorly suited for high throughput applications. Therefore, we have designed Griddent, a user-friendly platform with the capability of generating controllable and reversible gradients in a 3D microenvironment. Our platform consists of an array of 32 microfluidic chambers connected to a 384 well-array through a diffusion port at the bottom of each

SUBMITTER: Sanchez-de-Diego C 

PROVIDER: S-EPMC10492845 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature