N⁶-Methyladenosine-Modified ATP8B1-AS1 Exerts Oncogenic Roles in Hepatocellular Carcinoma via Epigenetically Activating MYC.

ABSTRACT:

Purpose

N⁶-methyladenosine (m⁶A) modification has shown critical roles in regulating mRNA fate. Non-coding RNAs also have important roles in various diseases, including hepatocellular carcinoma (HCC). However, the potential influences of m⁶A modification on non-coding RNAs are still unclear. In this study, we identified a novel m⁶A-modified ATP8B1-AS1 and aimed to investigate the effects of m⁶A on the expression and role of ATP8B1-AS1 in HCC.

Methods

qPCR was performed to measure the expression of related genes. The correlation between gene expression and prognosis was analyzed using public database. m⁶A modification level was measured using MeRIP and single-base elongation- and ligation-based qPCR amplificati

SUBMITTER: Tan C 

PROVIDER: S-EPMC10493143 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature