C-terminal sequence stability profiling in Saccharomyces cerevisiae reveals protective protein quality control pathways.

ABSTRACT: Protein quality control (PQC) mechanisms are essential for degradation of misfolded or dysfunctional proteins. An essential part of protein homeostasis is recognition of defective proteins by PQC components and their elimination by the ubiquitin-proteasome system, often concentrating on protein termini as indicators of protein integrity. Changes in amino acid composition of C-terminal ends arise through protein disintegration, alternative splicing, or during the translation step of protein synthesis from premature termination or translational stop-codon read-through. We characterized reporter protein stability using light-controlled exposure of the random C-terminal peptide collection (CtPC) in budding yeast revealing stabilizing and destabilizing features of amino acids at positions -5 to

SUBMITTER: Hasenjager S 

PROVIDER: S-EPMC10493509 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature