The tomato chloroplast stromal proteome compendium elucidated by leveraging a plastid protein-localization prediction Atlas.

ABSTRACT: Tomato (Solanum lycopersicum) is a model species for studying fruit development, wounding, herbivory, and pathogen attack. Despite tomato's world-wide economic importance and the role of chloroplasts as metabolic hubs and integrators of environmental cues, little is known about the stromal proteome of tomato. Using a high-yielding protocol for chloroplast and stromal protein isolation, MudPIT nano-LC-MS/MS analyses, a robust in-house protein database (the Atlas) for predicting the plastid localization of tomato proteins, and rigorous selection criteria for inclusion/exclusion in the stromal proteome, we identified 1,278 proteins of the tomato stromal proteome. We provide one of the most robust stromal proteomes available to date with empirical evidence for 545 and 92 proteins not pr

SUBMITTER: Bhattacharya O 

PROVIDER: S-EPMC10493611 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature