Megf10-related engulfment of excitatory postsynapses by astrocytes following severe brain injury.

ABSTRACT:

Aims

To investigate astrocyte-related phagocytosis of synapses in the ipsilateral hippocampus after traumatic brain injury (TBI).

Methods

We performed controlled cortical impact to simulate TBI in mice. Seven days postinjury, we performed cognitive tests, synapse quantification, and examination of astrocytic phagocytosis in association with Megf10 expression.

Results

During the subacute stage post-TBI, we found a reduction in excitatory postsynaptic materials in the ipsilateral hippocampus, which was consistent with poor performance in the cognitive test. The transcriptome data suggested that robust phagocytosis was responsible for this process. Coincidently, we identified phagocytic astrocytes containing secondary lysosomes that were wrapped around the synapses in th

SUBMITTER: Zhuang Y 

PROVIDER: S-EPMC10493650 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature