Super-high procoagulant activity of gecko thrombin: A gift from sky dragon.

ABSTRACT:

Aims

Gecko, the "sky dragon" named by Traditional Chinese Medicine, undergoes rapid coagulation and scarless regeneration following tail amputation in the natural ecology, providing a perfect opportunity to develop the efficient and safe drug for blood clotting. Here, gecko thrombin (gthrombin) was recombinantly prepared and comparatively studied on its procoagulant activity.

Methods

The 3D structure of gthrombin was constructed using the homology modeling method of I-TASSER. The active gthrombin was prepared by the expression of gecko prethrombin-2 in 293 T cells, followed by purification with Ni²⁺ -chelating column chromatography prior to activation by snake venom-derived Ecarin. The enzymatic activities of gthrombin were assayed by hydrolysis of synthetic subst

SUBMITTER: Liang H 

PROVIDER: S-EPMC10493662 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature