ARID4B loss leads to activated STAT1-dependent interferon pathway in mouse embryonic stem cells and during meso/endodermal differentiation.

ABSTRACT:

Objective

Proper deactivation of the pluripotency network and activation of a lineage-specific gene expression program are critical for mouse embryonic stem cell (mESC) differentiation. This is achieved by the coordinated action of transcription and chromatin factors. Our previous work identified ARID4B as a critical chromatin factor for mesoderm and endoderm differentiation. As part of a histone deacetylase complex, ARID4B plays a role in transcriptional suppression of its direct targets. Here, we investigated the mechanism of ARID4B function in mESC differentiation by focusing on genes and pathways that are upregulated in its absence.

Material and methods

We analyzed transcriptomic results of wild-type and arid4bΔ endoderm or mesoderm differentiated cells through integrati

SUBMITTER: Terzi Cizmecioglu N 

PROVIDER: S-EPMC10493817 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature