Loss of West Nile virus genetic diversity during mosquito infection due to species-dependent population bottlenecks.

ABSTRACT: Vector competence (VC) refers to the efficiency of pathogen transmission by vectors. Each step in the infection of a mosquito vector constitutes a barrier to transmission that may impose bottlenecks on virus populations. West Nile virus (WNV) is maintained by multiple mosquito species with varying VC. However, the extent to which bottlenecks and VC are linked is poorly understood. Similarly, quantitative analyses of mosquito-imposed bottlenecks on virus populations are limited. We used molecularly barcoded WNV to quantify tissue-associated population bottlenecks in three variably competent WNV vectors. Our results confirm strong population bottlenecks during mosquito infection that are capable of dramatically reshaping virus population structure in a non-selective manner. In addition, we f

SUBMITTER: Fitzmeyer EA 

PROVIDER: S-EPMC10494182 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature