CRISPR/Cas9-mediated editing of PHYTOENE DESATURASE gene in onion (Allium cepa L.).

ABSTRACT:

Introduction

Clustered regularly interspaced short palindromic repeats (CRISPR)/ CRISPR-associated protein 9 (Cas9) is a precise genome editing tool used to introduce genetic modifications in a wide range of crop species. Thus far, there is no report of CRISPR/Cas9-mediated genome editing in onions (Allium cepa L.).

Methods

In the present study, we targeted two exons of the gene coding for Phytoene desaturase (AcPDS) in onion cv. Bhima Super. The sgRNA-carrying constructs were co-cultivated with 8-week-old embryogenic calli using an Agrobacterium-mediated transformation protocol and incubated on the media without hygromycin B selection.

Results and discussion

Out of the total 617 co-cultivated calli, 21 (3.4%) regenerated shoots exhibited three di

SUBMITTER: Mainkar P 

PROVIDER: S-EPMC10494252 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature