Single-cell profiling of murine bladder cancer identifies sex-specific transcriptional signatures with prognostic relevance.

ABSTRACT: Bladder cancer (BLCA) is more common in men but more aggressive in women. Sex-based differences in cancer biology are commonly studied using a murine model with BLCA generated by N-butyl-N-(4-hydroxybutyl)-nitrosamine (BBN). While tumors in the BBN model have been profiled, these profiles provide limited information on the tumor microenvironment. Here, we applied single-cell RNA sequencing to characterize cell-type specific transcriptional differences between male and female BBN-induced tumors. We found proportional and gene expression differences in epithelial and non-epithelial subpopulations between male and female tumors. Expression of several genes predicted sex-specific survival in several human BLCA datasets. We identified novel and clinically relevant sex-specific transcriptional s

SUBMITTER: Abdel-Hafiz HA 

PROVIDER: S-EPMC10494466 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature