Proteomic Study of Middle Ear Effusion and Its Clinical Application for Otitis Media with Effusion.

ABSTRACT:

Background

Proteins found in middle ear effusion play crucial roles in the physiological and pathological processes of otitis media with effusion (OME), influencing the etiology and clinical characteristics of this disease. The qualitative and quantitative composition of these proteins depending on the underlying pathogenesis of middle ear effusion. Understanding their physiological and pathological functions is of great importance.

Methods

We collected samples from 19 volunteers diagnosed with OME. After offline separation using high-pH reversed-phase liquid chromatography (RPLC), the pooled sample was subjected to LC-MS/MS analysis to obtain a comprehensive profile of the OME proteome. Functional analysis was performed using Gene Ontology (GO), Kyoto Encyclopedia of Genes

SUBMITTER: Wang X 

PROVIDER: S-EPMC10494926 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature