Patch grafting of organoids of stem/progenitors into solid organs can correct genetic-based disease states.

ABSTRACT: Patch grafting, a novel strategy for transplantation of stem/progenitor organoids into porcine livers, has been found successful also for organoid transplantation into other normal or diseased solid organs in pigs and mice. Each organoid contained ∼100 cells comprised of biliary tree stem cells (BTSCs), co-hepato/pancreatic stem/progenitors, and partnered with early lineage stage mesenchymal cells (ELSMCs), angioblasts and precursors to endothelia and stellate cells. Patch grafting enabled transplantation into livers or pancreases of ≥10^8th (pigs) or ≥10^6th-7th (mice) organoids/patch. Graft conditions fostered expression of multiple matrix-metalloproteinases (MMPs), especially secretory isoforms, resulting in transient loss of the organ's matrix-dictated histological

SUBMITTER: Zhang W 

PROVIDER: S-EPMC10495116 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature