Method for Visualization of Emergency Granulopoiesis in the Zebrafish Embryo.

ABSTRACT: Emergency granulopoiesis (EG) is a response to severe inflammation in which increased neutrophils are generated in the hematopoietic tissue. Photolabeling is utilized to distinguish newly developed neutrophils from existing neutrophils. However, this technique requires a strong laser line and labels subsets of the existing neutrophils. Here we create a transgenic zebrafish line that expresses a time-dependent switch from green fluorescent protein (GFP) to red fluorescent protein (RFP) in neutrophils, which allows quantification of EG using simple GFP/RFP ratiometric imaging.

SUBMITTER: Syahirah R 

PROVIDER: S-EPMC10495196 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature