H.O.S.T.: Hemoglobin microbubble-based Oxidative stress Sensing Technology.

ABSTRACT: In this work, we discuss the development of H.O.S.T., a novel hemoglobin microbubble-based electrochemical biosensor for label-free detection of Hydrogen peroxide (H₂O₂) towards oxidative stress and cancer diagnostic applications. The novelty of the constructed sensor lies in the use of a sonochemically prepared hemoglobin microbubble capture probe, which allowed for an extended dynamic range, lower detection limit, and enhanced resolution compared to the native hemoglobin based H₂O₂ biosensors. The size of the prepared particles Hemoglobin microbubbles was characterized using Coulter Counter analysis and was found to be 4.4 microns, and the morphology of these spherical microbubbles was shown using Brightfield microscopy. The binding chemistry o

SUBMITTER: Ganguly A 

PROVIDER: S-EPMC10495409 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature