High throughput detection of capillary stalling events with Bessel beam two-photon microscopy.

ABSTRACT:

Significance

Brief disruptions in capillary flow, commonly referred to as capillary "stalling," have gained interest recently for their potential role in disrupting cerebral blood flow and oxygen delivery. Approaches to studying this phenomenon have been hindered by limited volumetric imaging rates and cumbersome manual analysis. The ability to precisely and efficiently quantify the dynamics of these events will be key in understanding their potential role in stroke and neurodegenerative diseases, such as Alzheimer's disease.

Aim

Our study aimed to demonstrate that the fast volumetric imaging rates offered by Bessel beam two-photon microscopy combined with improved data analysis throughput allows for faster and more precise measurement of capillary stall dynamics.

Results

SUBMITTER: Giblin J 

PROVIDER: S-EPMC10495839 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature