Epigenetic status of FBXW7 gene and its role in Ovarian cancer pathogenesis.

ABSTRACT:

Background

Chromatin immunoprecipitation (ChIP) analysis revealed that the FBXW7 gene and the long non-coding RNA (LINC01588) are potential candidates in epithelial ovarian cancer (EOC) pathogenesis. However, their exact role in EOC is not yet known. Thus, the present study sheds light on the impact of the mutations/ methylation status of the FBXW7 gene.

Materials and methods

We used public databases to assess the correlation between mutations/ methylation status and the FBXW7 expression. Furthermore, we performed Pearson's correlation analysis between the FBXW7 gene and LINC01588. We performed gene panel exome sequencing and Methylation-specific PCR (MSP) in HOSE 6-3, MCAS, OVSAHO, and eight EOC patients' samples to validate the bioinformatics results.

Results

The FBX

SUBMITTER: Al Hinai M 

PROVIDER: S-EPMC10495899 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature