Blue-green fluorescence during hypersensitive cell death arises from phenylpropanoid deydrodimers.

ABSTRACT: Infection of Arabidopsis with avirulent Pseudomonas syringae and exposure to nitrogen dioxide (NO₂) both trigger hypersensitive cell death (HCD) that is characterized by the emission of bright blue-green (BG) autofluorescence under UV illumination. The aim of our current work was to identify the BG fluorescent molecules and scrutinize their biosynthesis, localization, and functions during the HCD. Compared with wild-type (WT) plants, the phenylpropanoid-deficient mutant fah1 developed normal HCD except for the absence of BG fluorescence. Ultrahigh resolution metabolomics combined with mass difference network analysis revealed that WT but not fah1 plants rapidly accumulate dehydrodimers of sinapic acid, sinapoylmalate, 5-hydroxyferulic acid, and 5-hydroxyferu

SUBMITTER: Kanawati B 

PROVIDER: S-EPMC10496137 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature