The relationship between regulatory changes in cis and trans and the evolution of gene expression in humans and chimpanzees.

ABSTRACT:

Background

Comparative gene expression studies in apes are fundamentally limited by the challenges associated with sampling across different tissues. Here, we used single-cell RNA sequencing of embryoid bodies to collect transcriptomic data from over 70 cell types in three humans and three chimpanzees.

Results

We find hundreds of genes whose regulation is conserved across cell types, as well as genes whose regulation likely evolves under directional selection in one or a handful of cell types. Using embryoid bodies from a human-chimpanzee fused cell line, we also infer the proportion of inter-species regulatory differences due to changes in cis and trans elements between the species. Using the cis/trans inference and an analysis of transcription factor binding sites, we iden

SUBMITTER: Barr KA 

PROVIDER: S-EPMC10496171 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature