Murine leukemia virus (MLV) P50 protein induces cell transformation via transcriptional regulatory function.

ABSTRACT:

Background

The murine leukemia virus (MLV) has been a powerful model of pathogenesis for the discovery of genes involved in cancer. Its splice donor (SD')-associated retroelement (SDARE) is important for infectivity and tumorigenesis, but the mechanism remains poorly characterized. Here, we show for the first time that P50 protein, which is produced from SDARE, acts as an accessory protein that transregulates transcription and induces cell transformation.

Results

By infecting cells with MLV particles containing SDARE transcript alone (lacking genomic RNA), we show that SDARE can spread to neighbouring cells as shown by the presence of P50 in infected cells. Furthermore, a role for P50 in cell transformation was demonstrated by CCK8, TUNEL and anchorage-independent growth ass

SUBMITTER: Akkawi C 

PROVIDER: S-EPMC10496198 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature