Coupling of co-transcriptional splicing and 3' end Pol II pausing during termination in Arabidopsis.

ABSTRACT:

Background

In Arabidopsis, RNA Polymerase II (Pol II) often pauses within a few hundred base pairs downstream of the polyadenylation site, reflecting efficient transcriptional termination, but how such pausing is regulated remains largely elusive.

Result

Here, we analyze Pol II dynamics at 3' ends by combining comprehensive experiments with mathematical modelling. We generate high-resolution serine 2 phosphorylated (Ser2P) Pol II positioning data specifically enriched at 3' ends and define a 3' end pause index (3'PI). The position but not the extent of the 3' end pause correlates with the termination window size. The 3'PI is not decreased but even mildly increased in the termination deficient mutant xrn3, indicating 3' end pause is a regulatory step early during the terminat

SUBMITTER: Zhou S 

PROVIDER: S-EPMC10496290 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature