Injury-experienced satellite cells retain long-term enhanced regenerative capacity.

ABSTRACT:

Background

Inflammatory memory or trained immunity is a recently described process in immune and non-immune tissue resident cells, whereby previous exposure to inflammation mediators leads to a faster and stronger responses upon secondary challenge. Whether previous muscle injury is associated with altered responses to subsequent injury by satellite cells (SCs), the muscle stem cells, is not known.

Methods

We used a mouse model of repeated muscle injury, in which intramuscular cardiotoxin (CTX) injections were administered 50 days apart in order to allow for full recovery of the injured muscle before the second injury. The effect of prior injury on the phenotype, proliferation and regenerative potential of satellite cells following a second injury was examined in vitro and i

SUBMITTER: Morroni J 

PROVIDER: S-EPMC10496398 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature