Nanobiocatalysts with inbuilt cofactor recycling for oxidoreductase catalysis in organic solvents.

ABSTRACT: The major stumbling block in the implementation of oxidoreductase enzymes in continuous processes is their stark dependence on costly cofactors that are insoluble in organic solvents. We describe a chemical strategy that allows producing nanobiocatalysts, based on an oxidoreductase enzyme, that performs biocatalytic reactions in hydrophobic organic solvents without external cofactors. The chemical design relies on the use of a silica-based carrier nanoparticle, of which the porosity can be exploited to create an aqueous reservoir containing the cofactor. The nanoparticle core, possessing radial-centred pore channels, serves as a cofactor reservoir. It is further covered with a layer of reduced porosity. This layer serves as a support for the immobilisation of the selected enzyme yet allowi

SUBMITTER: Sahlin J 

PROVIDER: S-EPMC10496889 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature