Molecular characterization of MRSA collected during national surveillance between 2008 and 2019 in the Netherlands.

ABSTRACT:

Background

Although the Netherlands is a country with a low endemic level, methicillin-resistant Staphylococcus aureus (MRSA) poses a significant health care problem. Therefore, high coverage national MRSA surveillance has been in place since 1989. To monitor possible changes in the type-distribution and emergence of resistance and virulence, MRSA isolates are molecularly characterized.

Methods

All 43,321 isolates from 36,520 persons, collected 2008-2019, were typed by multiple-locus variable number tandem repeats analysis (MLVA) with simultaneous PCR detection of the mecA, mecC and lukF-PV genes, indicative for PVL. Next-generation sequencing data of 4991 isolates from 4798 persons were used for whole genome multi-locus sequence typing (wgMLST) and identification of resista

SUBMITTER: Schouls LM 

PROVIDER: S-EPMC10497500 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature