A Lassa virus mRNA vaccine confers protection but does not require neutralizing antibody in a guinea pig model of infection.

ABSTRACT: Lassa virus is a member of the Arenaviridae family, which causes human infections ranging from asymptomatic to severe hemorrhagic disease with a high case fatality rate. We have designed and generated lipid nanoparticle encapsulated, modified mRNA vaccines that encode for the wild-type Lassa virus strain Josiah glycoprotein complex or the prefusion stabilized conformation of the Lassa virus glycoprotein complex. Hartley guinea pigs were vaccinated with two 10 µg doses, 28 days apart, of either construct. Vaccination induced strong binding antibody responses, specific to the prefusion conformation of glycoprotein complex, which were significantly higher in the prefusion stabilized glycoprotein complex construct group and displayed strong Fc-mediated effects. However, Lassa virus-neutralizin

SUBMITTER: Ronk AJ 

PROVIDER: S-EPMC10497546 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature