Metabolic heterogeneity of tissue-resident macrophages in homeostasis and during helminth infection.

ABSTRACT: Tissue-resident macrophage populations constitute a mosaic of phenotypes, yet how their metabolic states link to the range of phenotypes and functions in vivo is still poorly defined. Here, using high-dimensional spectral flow cytometry, we observe distinct metabolic profiles between different organs and functionally link acetyl CoA carboxylase activity to efferocytotic capacity. Additionally, differences in metabolism are evident within populations from a specific site, corresponding to relative stages of macrophage maturity. Immune perturbation with intestinal helminth infection increases alternative activation and metabolic rewiring of monocyte-derived macrophage populations, while resident TIM4⁺ intestinal macrophages remain immunologically and metabolically hyporesponsive.

SUBMITTER: Heieis GA 

PROVIDER: S-EPMC10497597 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature