SHIELD: a platform for high-throughput screening of barrier-type DNA elements in human cells.

ABSTRACT: Chromatin boundary elements contribute to the partitioning of mammalian genomes into topological domains to regulate gene expression. Certain boundary elements are adopted as DNA insulators for safe and stable transgene expression in mammalian cells. These elements, however, are ill-defined and less characterized in the non-coding genome, partially due to the lack of a platform to readily evaluate boundary-associated activities of putative DNA sequences. Here we report SHIELD (Site-specific Heterochromatin Insertion of Elements at Lamina-associated Domains), a platform tailored for the high-throughput screening of barrier-type DNA elements in human cells. SHIELD takes advantage of the high specificity of serine integrase at heterochromatin, and exploits the natural heterochromatin spreadin

SUBMITTER: Zhang M 

PROVIDER: S-EPMC10497619 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature