Measuring ADAMTS-13 activity to diagnose thrombotic thrombocytopenic purpura: a novel, fast fiber-optic surface plasmon resonance immunoassay.

ABSTRACT:

Background

Thrombotic thrombocytopenic purpura (TTP) is characterized by severe ADAMTS-13 activity deficiency (<10%). Diagnostic testing is challenging because of unavailability, high cost, and expert technician requirement of ADAMTS-13 enzyme assays. Cost-effective, automated fiber-optic surface plasmon resonance (FO-SPR) platforms show potential for developing diagnostic tests. Yet, FO-SPR has never been explored to measure enzymatic activities.

Objectives

To develop an easy-to-use ADAMTS-13 activity assay utilizing optical fibers to rapidly diagnose TTP.

Methods

The ADAMTS-13 activity assay was designed and optimized using FO-SPR technology based on a previously described enzyme-linked immunosorbent assay setup. A calibration curve was generated to quantify ADAMTS-

SUBMITTER: Bonnez Q 

PROVIDER: S-EPMC10497779 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature