Mechanism of RBBP8-mediated homologous recombination repair in gastric cancer synthetic lethal.

ABSTRACT:

Background

It is of great clinical significance to further explore new strategies and potential combined therapeutic targets for gastric cancer. This study aimed to investigate the synthetic lethal effect of RBBP8 molecular intervention combined with a poly ADP ribose polymerase (PARP) inhibitor in non-BRCA mutant gastric cancer and clarify the mechanism by which RBBP8 regulates homologous recombination repair.

Methods

The role of RBBP8 in DNA damage repair was observed using bioinformatic analysis, western blot analysis, and immunofluorescence. The synthetic lethal effect was verified using 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt (MTS)and flow cytometry apoptosis experiments.

Results

Among the patients with

SUBMITTER: Yu Y 

PROVIDER: S-EPMC10497805 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature