Deletion of enzymes for de novo NAD⁺ biosynthesis accelerated ovarian aging.

ABSTRACT: Recent advances highlight the pivotal role of nicotinamide adenine dinucleotide (NAD⁺ ) in ovarian aging. However, the roles of de novo NAD⁺ biosynthesis on ovarian aging are still unknown. Here, we found that genetic ablation of Ido1 (indoleamine-2,3-dioxygenase 1) or Qprt (Quinolinate phosphoribosyl transferase), two critical genes in de novo NAD⁺ biosynthesis, resulted in decreased ovarian NAD⁺ levels in middle-aged mice, leading to subfertility, irregular estrous cycles, reduced ovarian reserve, and accelerated aging. Moreover, we observed impaired oocyte quality, characterized by increased reactive oxygen species and spindle anomalies, which ultimately led to reduced fertilization ability and impaired early embryonic development. A transcrip

SUBMITTER: Yang Q 

PROVIDER: S-EPMC10497836 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature