Stability of the Fecal and Oral Microbiome over 2 Years at -80°C for Multiple Collection Methods.

ABSTRACT:

Background

In prospective cohorts, biological samples are generally stored over long periods before an adequate number of cases have accrued. We investigated the impact of sample storage at -80°C for 2 years on the stability of the V4 region of the 16S rRNA gene across seven different collection methods (i.e., no additive, 95% ethanol, RNAlater stabilization solution, fecal occult blood test cards, and fecal immunochemical test tubes for feces; OMNIgene ORAL tubes and Scope mouthwash for saliva) among 51 healthy volunteers.

Methods

Intraclass correlation coefficients (ICC) were calculated for the relative abundance of the top three phyla, the 20 most abundant genera, three alpha-diversity metrics, and the first principal coordinates of three beta-diversity matrices.

Resul

SUBMITTER: Zouiouich S 

PROVIDER: S-EPMC10498478 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature