Analysis of acid-tolerance mechanism based on membrane microdomains in Saccharomyces cerevisiae.

ABSTRACT:

Background

Saccharomyces cerevisiae has been used in the biosynthesis of acid products such as organic acids owing to its acid tolerance. Improving the acid tolerance of S. cerevisiae is beneficial for expanding its application range. Our previous study isolated the TAMC strain that was tolerant to a pH 2.3 through adaptive laboratory evolution; however, its mechanism underlying tolerance to low pH environment remains unclear.

Results

In this study, through visual observation and order analysis of plasma membrane and membrane microdomains, we revealed that the membrane microdomains of TAMC strain play an indispensable role in acid tolerance. Transcriptomic analysis showed an increase in the expression of genes related to key components of membrane microdomains in TAMC strain

SUBMITTER: Lv X 

PROVIDER: S-EPMC10498586 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature