Stochastic assembly of biomacromolecular complexes: impact and implications on charge interpretation in native mass spectrometry.

ABSTRACT: Native mass spectrometry is a potent method for characterizing biomacromolecular assemblies. A critical aspect to extracting accurate mass information is the correct inference of the ion ensemble charge states. While a variety of experimental strategies and algorithms have been developed to facilitate this, virtually all approaches rely on the implicit assumption that any peaks in a native mass spectrum can be directly attributed to an underlying charge state distribution. Here, we demonstrate that this paradigm breaks down for several types of macromolecular protein complexes due to the intrinsic heterogeneity induced by the stochastic nature of their assembly. Utilizing several protein assemblies of adeno-associated virus capsids and ferritin, we demonstrate that these particles can prod

SUBMITTER: Yin V 

PROVIDER: S-EPMC10498669 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature