SIRT3 Regulates Clearance of Apoptotic Cardiomyocytes by Deacetylating Frataxin.

ABSTRACT:

Background

Efferocytosis is an activity of macrophages that is pivotal for the resolution of inflammation in hypertension. The precise mechanism by which macrophages coordinate efferocytosis and internalize apoptotic cardiomyocytes remains unknown. The aim of this study was to determine whether SIRT3 (sirtuin-3) is required for both apoptotic cardiomyocyte engulfment and anti-inflammatory responses during efferocytosis.

Methods

We generated myeloid SIRT3 knockout mice and FXN (frataxin) knock-in mice carrying an acetylation-defective lysine to arginine K189R mutation (FXN^K189R). The mice were given Ang II (angiotensin II) infusion for 7 days. We analyzed cardiac macrophages' mitochondrial iron levels, efferocytosis activity, and phenotype both in vivo and in vitro

SUBMITTER: Gao J 

PROVIDER: S-EPMC10498872 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature