Proteomic Atlas of Atherosclerosis: The Contribution of Proteoglycans to Sex Differences, Plaque Phenotypes, and Outcomes.

ABSTRACT:

Background

Using proteomics, we aimed to reveal molecular types of human atherosclerotic lesions and study their associations with histology, imaging, and cardiovascular outcomes.

Methods

Two hundred nineteen carotid endarterectomy samples were procured from 120 patients. A sequential protein extraction protocol was employed in conjunction with multiplexed, discovery proteomics. To focus on extracellular proteins, parallel reaction monitoring was employed for targeted proteomics. Proteomic signatures were integrated with bulk, single-cell, and spatial RNA-sequencing data, and validated in 200 patients from the Athero-Express Biobank study.

Results

This extensive proteomics analysis identified plaque inflammation and calcification signatures, which were inversely corre

SUBMITTER: Theofilatos K 

PROVIDER: S-EPMC10498884 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature