Extensive rewiring of the gene regulatory interactions between in vitro-produced conceptuses and endometrium during attachment.

ABSTRACT: Pregnancy loss is a significant problem when embryos produced in vitro are transferred to a synchronized uterus. Currently, mechanisms that underlie losses of in vitro-produced embryos during implantation are largely unknown. We investigated this problem using cattle as a model of conceptus attachment by analyzing transcriptome data of paired extraembryonic membrane and endometrial samples collected on gestation days 18 and 25, which spans the attachment window in cattle. We identified that the transfer of an in vitro-produced embryo caused a significant alteration in transcript abundance of hundreds of genes in extraembryonic and endometrial tissues on gestation days 18 and 25, when compared to pregnancies initiated by artificial insemination. Many of the genes with altered transcript abu

SUBMITTER: Biase FH 

PROVIDER: S-EPMC10498941 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature