In host evolution of beta lactam resistance during active treatment for Pseudomonas aeruginosa bacteremia.

ABSTRACT: Multidrug-resistant (MDR) Pseudomonas aeruginosa has been declared a serious threat by the United States Centers for Disease Control and Prevention. Here, we used whole genome sequencing (WGS) to investigate recurrent P. aeruginosa bloodstream infections in a severely immunocompromised patient. The infections demonstrated unusual, progressive increases in resistance to beta lactam antibiotics in the setting of active treatment with appropriate, guideline-directed agents. WGS followed by comparative genomic analysis of isolates collected over 44 days demonstrated in host evolution of a single P. aeruginosa isolate characterized by stepwise acquisition of two de-novo genetic resistance mechanisms over the course of treatment. We found a novel deletion affecting th

SUBMITTER: Spottiswoode N 

PROVIDER: S-EPMC10499174 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature