Function and regulation of a steroidogenic CYP450 enzyme in the mitochondrion of Toxoplasma gondii.

ABSTRACT: As an obligate intracellular parasite, Toxoplasma gondii must import essential nutrients from the host cell into the parasitophorous vacuole. We previously reported that the parasite scavenges cholesterol from host endocytic organelles for incorporation into membranes and storage as cholesteryl esters in lipid droplets. In this study, we have investigated whether Toxoplasma utilizes cholesterol as a precursor for the synthesis of metabolites, such as steroids. In mammalian cells, steroidogenesis occurs in mitochondria and involves membrane-bound type I cytochrome P450 oxidases that are activated through interaction with heme-binding proteins containing a cytochrome b5 domain, such as members of the membrane-associated progesterone receptor (MAPR) family. Our LC-MS targeted lipidomics detec

SUBMITTER: Asady B 

PROVIDER: S-EPMC10499268 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature