CRISPR-based engineering of RNA viruses.

ABSTRACT: CRISPR RNA-guided endonucleases have enabled precise editing of DNA. However, options for editing RNA remain limited. Here, we combine sequence-specific RNA cleavage by CRISPR ribonucleases with programmable RNA repair to make precise deletions and insertions in RNA. This work establishes a recombinant RNA technology with immediate applications for the facile engineering of RNA viruses.

SUBMITTER: Nemudryi A 

PROVIDER: S-EPMC10499312 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature