Inferring and perturbing cell fate regulomes in human brain organoids.

ABSTRACT: Self-organizing neural organoids grown from pluripotent stem cells^1-3 combined with single-cell genomic technologies provide opportunities to examine gene regulatory networks underlying human brain development. Here we acquire single-cell transcriptome and accessible chromatin data over a dense time course in human organoids covering neuroepithelial formation, patterning, brain regionalization and neurogenesis, and identify temporally dynamic and brain-region-specific regulatory regions. We developed Pando-a flexible framework that incorporates multi-omic data and predictions of transcription-factor-binding sites to infer a global gene regulatory network describing organoid development. We use pooled genetic perturbation with single-cell transcriptome readout to assess transcrip

SUBMITTER: Fleck JS 

PROVIDER: S-EPMC10499607 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature