Spatial proteomics landscape and immune signature analysis of renal sample of lupus nephritis based on laser-captured microsection.

ABSTRACT:

Objective

We aimed to reveal a spatial proteomic and immune signature of kidney function regions in lupus nephritis (LN).

Material and methods

The laser capture microdissection (LCM) was used to isolate the glomerulus, tubules, and interstitial of the kidney from paraffin samples. The data-independent acquisition (DIA) method was used to collect proteomics data. The bioinformatic analysis was performed.

Results

A total of 49,658 peptides and 4056 proteins were quantitated. Our results first showed that a high proportion of activated NK cells, naive B cells, and neutrophils in the glomerulus, activated NK cells in interstitial, and resting NK cells were accumulated in tubules in LN. The immune-related function analysis of differential expression proteins in different r

SUBMITTER: Zheng F 

PROVIDER: S-EPMC10499763 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature